nlp-22 is expressed in dynamic fashion in the RIA interneurons. (a) mRNA expression during larval development. Expression levels cycle in synchrony with each lethargus stage. Yellow circles depict each lethargus stage, as defined by cessation of feeding of the animals (Biological replicates per time point ≥3, technical replicates = 2 for each biological replicate; Error bars represent s.e.m.). (b) nlp-22 expression is increased in response to lin-42 over-expression during the adult stage. (*p<0.05, ** p<0.005, Student’s two-tailed t-Test, Biological replicates per condition ≥5, technical replicates = 2 for each biological replicate; Error bars represent s.e.m.). (c) An nlp-22 transcriptional GFP reporter is expressed in the RIA neurons (arrow). Intestinal auto-fluorescence is also observed (arrow head). Scale Bar = 5μM.