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. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Nat Commun. 2013;4:2846. doi: 10.1038/ncomms3846

FIGURE 2.

FIGURE 2

NLP-22 induces behavioral quiescence. (a) Gene and protein structure of nlp-22 including over-expression construct. 2.5 hours after nlp-22 induction, animals show reduced movement (b) and feeding (c) relative to control animals over-expressing GFP (strain: TJ375). (c) Removing the signal sequence, or mutating the KR or FRPG eliminates the ability of NLP-22 to reduce pumping rate (**p<0.0001, Student’s two-tailed t-Test, N≥20). (d) Animals over-expressing nlp-22 cease pumping, but recover 9 hours after heat-shock (*p<0.001, **p<0.0001, Fisher’s exact test, N≥20 animals, 2 trials; Error bars represent s.e.m.).