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. Author manuscript; available in PMC: 2014 Jun 4.
Published in final edited form as: Nat Commun. 2013;4:2846. doi: 10.1038/ncomms3846

FIGURE 3.

FIGURE 3

Quiescence induced by nlp-22 OE resembles lethargus quiescence. (a) Animals that over-express nlp-22 (strain: NQ251) are less responsive to chemical (1-octanol) and optical (blue light) stimulation than control animals that over-express GFP (strain: TJ375) (**p<0.0001, Student’s two-tailed t-Test, N≥20; Error bars represent s.e.m.). (b) Over-expressing nlp-22 via a mild heat shock, such that full quiescence is not induced, results in increased backwards movement. White, dark gray, and light gray denote forward, backwards, and no movement, respectively. The average fraction of time spent moving forward or moving backward in a 120s window for nlp-22 over-expressing animals is significantly different from WT worms (*p<0.001. **p<0.0001, Student’s two-tailed t-Test, N=20). In addition to the bias for backwards motion, nlp-22 over-expressing animals also moved significantly less than WT animals (**p<0.0001, Student’s two-tailed t-Test, N=20).