Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Nov 17;20(12):1450–1457. doi: 10.1038/nsmb.2704

Figure 4.

Figure 4

Under C complex conditions, the pre-mRNA accesses dynamic and stabilized high-FRET states. (a) FRET probability distribution of the raw single molecule FRET trajectories upon the addition of Prp2, Spp2, Cwc25, and ATP to the Bact complex. (b) Representative single molecule FRET trajectories of molecules from the C complex condition with the raw donor (Cy3, green), acceptor (Cy5, red), FRET (black) trajectories, and idealized HMM models (cyan). (c) TODP generated from the idealized HMM for molecules in the C complex condition. L1, L2, M, or H refers to the four states resulting from clustering analysis.