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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Nat Struct Mol Biol. 2013 Nov 17;20(12):1450–1457. doi: 10.1038/nsmb.2704

Figure 5.

Figure 5

Cwc25 enhances the first step of splicing by stabilizing the H state. (a) A comparison of the aggregate molecular behavior before (B* condition) and after (C condition) Cwc25 addition through post-synchronized histograms (PSH) with all trajectories synchronized to start from either the M (top) or H state (bottom). (b) A comparison of the rate constants of the observable transitions under B* and C conditions. The thickness of arrows corresponds to the relative rate constants. (c) Representative single molecule FRET trajectories showing transition dynamics from the same molecules imaged before (B* condition) and after (C condition) Cwc25 addition with the raw donor (Cy3, green), acceptor (Cy5, red), and FRET (black) trajectories. The axis breaks represent 10 min of incubation after Cwc25 addition.