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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Anal Chem. 2013 Oct 28;85(22):10.1021/ac4020098. doi: 10.1021/ac4020098

Figure 2.

Figure 2

(a) Microfluidic device schematic. Solutions introduced into the two inlets are mixed while traversing the serpentine channels to establish a stable concentration gradient across the observation channel. While a gradient of fMLP is established by introducing media into the inlet 1 and fMLP solution into the inlet 2, a competing gradient of LTB4-fMLP is established by introducing LTB4 solution into the inlet 1 and fMLP solution into the inlet 2. (b) Neutrophils on a fibronectin-coated surface in the observation channel. (c) Trajectories of 20 representative control neutrophils (unactivated) within the fMLP gradient. (d) Neutrophils on an endothelial cell-coated surface in the observation channel. (e) Trajectories of 20 representative control neutrophils (unactivated) within the fMLP gradient.