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. Author manuscript; available in PMC: 2013 Dec 18.
Published in final edited form as: J Immunol. 2010 Jan 25;184(5):10.4049/jimmunol.0901022. doi: 10.4049/jimmunol.0901022

Figure 5.

Figure 5

Age-associated alteration in Toll-like receptor (TLR)–induced cytokine production in purified DCs isolated using magnetic bead sorting in 6 older, compared to 7 young adults two years after the original study. A. Age-associated decrease (n=13) in IL-12 (p40,) IL-6 and TNF-α production in mDCs (as measured by intracellular cytokine staining) following stimulation with indicated TLR ligands. B. Age-associated decrease in IFN-α and TNF-α production in pDCs (n=13) following stimulation with R848 and CpG. The mean ± SEM percent positive difference in intracellular cytokine production between samples stimulated with the indicated TLR ligand and unstimulated samples are depicted; P values after adjustment for covariates are indicated: #<0.01, $<0.05. C and D. Representative examples of TLR ligand induced cytokine production, depicted here TNF-α and IL-6 production in 6 young (Panel A) and 6 older adults (Panel B) after stimulation with TLR7/8 ligand R848.