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. Author manuscript; available in PMC: 2013 Dec 18.
Published in final edited form as: Cancer. 2012 Aug 22;119(3):10.1002/cncr.27725. doi: 10.1002/cncr.27725

Figure 3.

Figure 3

(A) HOXB7 expression was determined by quantitative PCR in PDAC cell lines following transfection with control small interfering RNA (siRNA), HOXB7 siRNA #1, or HOXB7 siRNA #2, as well as (B) parallel western blots. Corresponding in vitro assays are shown for (C) cell growth by MTT, (D) cell proliferation by bromodeoxyuridine (BrdU) incorporation, (E) invasiveness through Matrigel-coated transwell membrane inserts, or (F) migration through uncoated transwell membrane inserts toward chemoattractant. (G) Migration and matrigel invasion assays and western blots are shown with MiaPaCa-2 cells stably overexpressing GFP or HOXB7. Each sample condition was evaluated in triplicate or quadruplicate, with all assays repeated at least twice. Similar results were obtained using HOXB7 siRNA #3 (data not shown). *P < .05, **P < .005. Abbreviations: mRNA exp., messenger RNA expression; WB, western blot.