Table 1.
Effects of ND-AMB on serum chemistries in mice
| Treatment | Dose AMB (mg/kg) | Survival | Elevated kidney markers | Creat (mg/dL) mean (SD) | BUN (mg/dL) mean (SD) | ALT (U/L) mean (SD) | AST (U/L) mean (SD) |
|---|---|---|---|---|---|---|---|
| Baseline (D5W) | – | 5/5 | NA | 0.12 (0.01) | 20.5 (2.1) | 34.1 (2.7) | 104 (44) |
| ND-AMB35C | 2 | 5/5 | 0/5 | 0.1 (0.01) | 21.6 (1.2) | 32 (11) | 88.8 (40) |
| ND-AMB35C | 3 | 5/5 | 3/5 | 0.17 (0.09) | 60.3 (32.8) | 71 (83) | 142 (138) |
| ND-AMB35C | 4 | 4/5 | 3/4 | 0.41 (0.32) | 128 (75.1) | 45 (12) | 185 (57) |
| ND-AMB55C | 4 | 5/5 | 0/5 | 0.07 (0.02) | 22 (2.6) | 73 (37) | 284 (133) |
| ND-AMB55C | 8 | 4/5 | 4/5 | 1.7 (0.83)** | 330 (120)** | 129 (31)* | 382 (61)* |
| ND-AMB72C | 10 | 5/5 | 0/5 | 0.13 (0.04) | 22.8 (5.1) | 69 (19) | 160 (130) |
| ND-AMB72C | 12 | 5/5 | 0/5 | 0.08 (0.04) | 23 (10.3) | 135 (64) | 219 (115) |
| ND-AMB72C | 15 | 5/5 | 3/5 | 0.39 (0.49) | 99.5 (127) | 56.5 (33) | 132 (121) |
| DOC-AMB | 2 | 2/3 | 0/2 | 0.07 (0.01) | 16.5 (5.1) | 83 (52) | 176 (102) |
| DOC-AMB | 3 | 2/3 | 0/2 | 0.51 (–) | 160.2 (–) | 66 (–) | 237 (–) |
Notes:
*
P<0.05
**
P<0.0001 compared to baseline by one-way analysis of variance.
Abbreviations: ND, NanoDisk; AMB, amphotericin B; NA, not applicable; Creat, creatinine; BUN, blood urea nitrogen; ALT, alanine aminotransferase; AST, aspartate aminotransferase; SD, standard deviation; DOC, deoxycholate.