Figure 7. Myocilin is part of a large complex of proteins bound to the cytoplasmic domain of GPR143.

Elution fractions 3-6, containing WT MYOC from GPR143:MBP column were pooled and maintained in PBS prior to sedimentation, or adjusted to 0.1% SDS or 500 mM KCl to determine the stability of the protein complex. Samples were then subjected to glycerol gradient sedimentation, SDS-PAGE and analyzed by western blot (panel A). Densitometric analysis of the relative MYOC band intensities in the various fractions is displayed in panel B. Parallel gradients were run with proteins of known molecular weights, which were visualized by silver stain (not shown). The peaks of elution profiles for the standards are indicated with arrows and corresponding molecular weight. Results shown are from a single experiment that is representative of 3 experiments in total.