Table I.
Sequences of primers employed for bisulfite sequencing and methylation-specific PCR.
| Region | Sequence | (Refs.) | |
|---|---|---|---|
| Bisulfite sequencing | |||
| DLK1-GTL2 | Outer | F: TGG GAA TTG GGG TAT TGT TTA T R: AAA CAA TTT AAC AAC AAC TTT CCT C |
(19) |
| Inner | F: GTT AAG AGT TTG TGG ATT TGT GAG AAA TG R: CTA AAA ATC ACC AAA ACC CAT AAA ATC AC |
✓ | |
| IGF2-H19 | Outer | F: AGG TGT TTT AGT TTT ATG GAT GAT GG R: TCC TAT AAA TAT CCT ATT CCC AAA TAA CC |
(15) |
| Inner | F: TGT ATA GTA TAT GGG TAT TTT TGG AGG TTT R: H19-OR was used |
(15) | |
| LIT1 | (One PCR) | F: GTG TTA IGG IGG TGG AGA TTT TGT R: AAC TIA AAACACIAACCAATTCTCTA |
(16) |
| PEG1 | Outer | F: TTG TTG TTG GTT AGT TTT GTA TGG TT R: AAA AAT AAC ACC CCC TCC TCA AAT |
(15) |
| Inner | F: PEG1-OF was used R: CCC AAA AAC AAC CCC AAC TC |
(15) | |
| SNRPN | Outer | F: GTG TTG TGG GGT TTT AGG GGT TTA G R: CTC CCC AAA CTA TCT CTT AAA AAA AAC C |
(17) |
| Inner | F: AGG GAG TTG GGA TTT TTG TAT TG R: SNRPN-OR was used |
(17) | |
| Methylation-specific PCR | |||
| DLK1-GTL2 | Methylated | F: TAT TTT AAG ATT GTT AGT TTT TTC GC R: AAA ACC CAA CCC AAT AAA CG |
✓ |
| Unmethylated | F: GTTT TA TTT TAA GAT TGT TAG TTT TTT R: CAA AAC CCA ACC CAA TAA ACA |
✓ |
✓, Primers designed in this study; Outer, outer primers; Inner, inner primers; F, forward; R, reverse.