Figure 3. Examples of mutant plant phenotypes with differing doses of EMS and developmental stages.

A. 145 mM EMS treated chlorophyll aberration, only observed in M2 145 mM population, and not observed across all sibling M2 seedlings B. 130 mM treated mutant seedling C. 115 mM EMS treated mature plants showing root variation within pot trial D. 145 mM treated dwarf, no seed produced E. control F–I. 145 mM mature plants showing mutant phenotypes not seen in control populations; variations to plant architecture, leaf width, length and color, plant vigor, panicle shape, seed production and synchrony of maturity, and inter-nodal span length I. Individuals with this plant architecture did not produce seed. Other novel phenotypes observed in the mutant population included rhizome production, crooked nodes, non-surviving dwarfs, and sectoring as variegated leaves.