Table 3. Summary of Ca²⁺ sensitivity and properties of WT troponin complex at 23°C at various conditions.

Condition	pCa50	Eff Kd (μM)	nH	n
Tn Complex	8.19±0.23	0.007±0.003	−0.44±0.02	11
Tn Complex + Tm	8.05±0.1	0.009±0.002	−0.47±0.03	11
Thin filaments no S1	5.93±0.15	1.2±0.4	0.93±0.08	7
Thin filaments + cycling 0.1∶1 S1	5.96±0.12	1.1±0.3	1.53±0.39	8
Thin filaments + cycling 1∶1 S1	5.82±0.1	1.5±0.3	1.26±0.20	5
Thin filaments + 0.01∶1 rigor S1	6.29±0.03	0.51±0.04	1.33±0.10	3
Thin filaments + 0.1∶1 rigor S1	6.41±0.10	0.39±0.09	1.51±0.31	3
Thin filaments + 1–1.5∶1 rigor S1	6.85±0.02	0.14±0.01	−0.73±0.02	10

Mean ± SEM.

n_H is the Hill coefficient.

n, represents number of individual curves.

Ratios represent the molar ratio of S1 to thin filament.