Table 3. Summary of Ca2+ sensitivity and properties of WT troponin complex at 23°C at various conditions.
Condition | pCa50 | Eff Kd (μM) | nH | n |
Tn Complex | 8.19±0.23 | 0.007±0.003 | −0.44±0.02 | 11 |
Tn Complex + Tm | 8.05±0.1 | 0.009±0.002 | −0.47±0.03 | 11 |
Thin filaments no S1 | 5.93±0.15 | 1.2±0.4 | 0.93±0.08 | 7 |
Thin filaments + cycling 0.1∶1 S1 | 5.96±0.12 | 1.1±0.3 | 1.53±0.39 | 8 |
Thin filaments + cycling 1∶1 S1 | 5.82±0.1 | 1.5±0.3 | 1.26±0.20 | 5 |
Thin filaments + 0.01∶1 rigor S1 | 6.29±0.03 | 0.51±0.04 | 1.33±0.10 | 3 |
Thin filaments + 0.1∶1 rigor S1 | 6.41±0.10 | 0.39±0.09 | 1.51±0.31 | 3 |
Thin filaments + 1–1.5∶1 rigor S1 | 6.85±0.02 | 0.14±0.01 | −0.73±0.02 | 10 |
Mean ± SEM.
nH is the Hill coefficient.
n, represents number of individual curves.
Ratios represent the molar ratio of S1 to thin filament.