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. Author manuscript; available in PMC: 2013 Dec 18.
Published in final edited form as: J Cell Biochem. 2012 Jan;113(1):10.1002/jcb.23333. doi: 10.1002/jcb.23333

Figure 1. FOXA1 is necessary for IGF-I-mediated regulation of target gene expression.

Figure 1

A) In silico analysis was performed comparing the top 50 IGF-I induced and top 50 IGF-I-repressed genes from a microarray previously reported from our lab with publically available FOXA1 ChIP-on-chip data. FOXA1 binding was assessed in the regions ranging from the transcriptional start site to −50kB and intronic sequences of each gene. A Fisher’s Exact Test was used to determine the significance and the confidence interval. B) MCF7 cells were transfected with non-specific siRNA (NS siRNA) or siRNA against FOXA1 (FOXA1 siRNA) followed by either maintenance in serum-free medium or stimulation with IGF-I (100ng/mL). Q-RT-PCR was used to calculate relative mRNA expression as fold change compared to serum-free conditions. The data are an average of three biological replicates ± SEM. A t-test analysis was performed in which the IGF-I treated group was compared to the serum-free group (* p<0.05).