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. Author manuscript; available in PMC: 2014 Oct 31.
Published in final edited form as: Cell Rep. 2013 Oct 31;5(2):10.1016/j.celrep.2013.09.024. doi: 10.1016/j.celrep.2013.09.024

Figure 3. PRR14 Colocalizes with HP1 on Anaphase Chromatin.

Figure 3

(A) HeLa cells stably expressing full length N-terminal GFP-tagged PRR14 were isolated. Cells were fixed, stained with anti-GFP and anti-Lamin A/C antibodies, and imaged using confocal microscopy. Merge 1 excludes the DAPI signal. Images are representative of the highly uniform behavior of the GFP-tagged PRR14 protein.

(B) Cells were prepared similarly to Panel A, except that the anti-LBR staining was compared to GFP-tagged PRR14.

(C) (Left) HeLa cells stably expressing both full length N-terminal GFP-tagged PRR14 and mRFP-tagged HP1α were isolated. Live cell confocal imaging is shown. (Right) HeLa cells stably expressing PRR14 N-GFP 1–135 were transiently transfected with an expression plasmid encoding mRFP-HP1α, and live cell confocal imaging is shown. Arrows indicate lack of PRR14 1–135 and HP1α colocalization only at the spindle midzone and midbody. See Figure S3.