Figure 1.

Clustering pattern of high-quality SNPs analyzed with GenomeStudio. All SNPs in the 240 genotypes assessed were grouped into three clusters: AA (red), AB (purple), and BB (blue). The normalized θ range was 0–0.2 and 0.8–1 for homozygous clusters AA and BB, respectively. The black datapoints were regarded as “no calls.” A) PZA-03707.2 represented high-quality SNPs corresponding to clear homozygous clusters (AA or BB). B) Datapoints falling within the range of the normalized θ values were manually adjusted into the respective clusters for SYN22774 to increase the calling accuracy. SNPs with inadequate cluster separation were deleted from the 56,110 SNP set.