Figure 3.

Inhibition of USP14 activity does not alter proteasome catalytic activity in the amygdala. In vitro proteasome activity was measured in amygdala lysates collected from naive animals (n = 4). Samples were incubated in DMSO, IU1 (100 μM, 200 μM, and 400 μM), βlac (150 μM), and IU1+βlac (200 μM + 150 μM and 400 μM + 150 μM) solutions for 30 min prior to the addition of the fluorogenic substrate. IU1 infusions did not alter the time-dependent degradation of the fluorogenic substrate. (*) P < 0.05.