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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Cell Biol. 2013 Sep 22;15(10):10.1038/ncb2843. doi: 10.1038/ncb2843

Figure 6.

Figure 6

Neither NG2-glia nor neurons contribute to iANBs. (a) A genetic approach to trace NG2-glia lineage. (b) Experimental design. (c,d) NG2-Cre-traced cells are NG2 cells, oligodendrocytes or their precursors, but not astrocytes or microglia. n.d., not detected. Data are presented as mean ± s.d. Mean is shown for n = 3 animals, scoring for each animal 250 or more YFP+ cells from 3 to 6 brain sections for each marker. (e) Immunofluorescence analysis showing none of the induced DCX+ cells is labelled by YFP in striatal regions injected with SOX2 virus. A higher-magnification view of the outlined area is shown in the right panel. (f,g) A genetic approach to trace neurons and their derivatives. (h,i) Neurons are permanently labelled by tamoxifen induced expression of tdT in PrP–CreERT;Rosa–tdT mice. Data are presented as mean ± s.d. Mean is shown for n = 3 animals, scoring for each animal 300 or more tdT+ cells from 3 to 6 brain sections for each marker. (j) SOX2-induced iANBs in the striatum are not labelled by tdT, indicating a non-neuronal origin. Scale bars, 20 μm.