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. Author manuscript; available in PMC: 2013 Dec 19.
Published in final edited form as: J Immunol. 2010 Jul 30;185(5):10.4049/jimmunol.1000761. doi: 10.4049/jimmunol.1000761

FIGURE 1.

FIGURE 1

Disruption of actin filaments in target cells prevents conjugate formation with NK cells. A, 721.221 cells pre-treated with DMSO carrier alone (filled circles), Latrunculin A (left side), or Jasplakinolide (right side) at indicated concentrations for 40 min at 37°C were tested for conjugate formation with resting NK cells. Bars indicate the SD (n = 3 individual NK cell donors in three independent experiments). B, 721.221 target cells treated as in A were mixed with resting NK cells and degranulation was measured after 1 h at 37°C by staining for CD107a at the surface of NK cells. Each symbol indicates one of the six (left side) or four (right side) individual NK cell donors. Six (left side) or four (right side) independent experiments were performed.