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. Author manuscript; available in PMC: 2013 Dec 19.
Published in final edited form as: J Immunol. 2010 Jul 30;185(5):10.4049/jimmunol.1000761. doi: 10.4049/jimmunol.1000761

FIGURE 2.

FIGURE 2

Disruption of actin filaments in 721.221 target cells inhibits polarization of cytolytic granules in NK cells. 721.221 cells incubated either with DMSO carrier (A, B, filled histograms) or with 3 μM (A) or 20 μM (B) Latrunculin A were mixed with NK cells for 20 min at 37°C. The cells were transferred to poly-D-lysine coated culture slides and incubated for 1 h at room temperature before fixation, permeabilization, and staining for perforin. Granule polarization is expressed as the fraction of cells in contact with perforin clustered at the NK-target cell interface. Bars indicate the SD (n = 3 individual donors in three independent experiments).