Figure 1.

(A) A toy GRN representing interactions between a set of genes/proteins. Arrow-headed edges represent activation and circle-headed edges represent inhibiting interactions. (B) MIS patters to push the systems into a steady state where T = 1. For every MIS vector there is at least one mutations that has to be present. The polarity of these mandatory mutations are indicated by red (knock-out) or blue (over-express) colors. In addition, compatible off-target mutations and their polarities (over-expression or knock-out) are also listed for every MIS vector. Genes correponding to white color in an MIS vector indicate the mutations in these genes have no effect on the corresponding MIS vector. Genes correponding to gray color in an MIS vector should not be perturbed in any polarity (either by knock-out or over-expression). Else the corresponding MIS vector may not be able to generate the desired steady state.