Figure 2.

Post-treatment with 25 mg/kg, but not 5 mg/kg, laquinimod (LQ) reduces cytokine production by peripheral immune cells in EAE-induced female mice. EAE was induced as in Figure 1. Female mice from the 5 mg/kg and 25 mg/kg LQ early post-treatment groups were used for cytokine analysis. On day 36, cytokine production by MOG 35–55 peptide-stimulated splenocytes was determined. No significant change in cytokine levels was observed with 5 mg/kg LQ early post-treatment. There was an overall decrease in cytokine levels in the 25 mg/kg LQ early post-treated as compared to the vehicle-treated EAE group. Error bars indicate variability of cytokine values for splenocytes between individual mice within a given treatment group, with n = 5–8 mice/group. Data are representative of experiments repeated twice (**P < 0.001, t-test).