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. 2014 Jan;95(1):83–93. doi: 10.1189/jlb.1112608

Figure 5. DAPK2 is a relevant PU.1 target during neutrophil differentiation of APL cells.

Figure 5.

(A–E) Ectopic expression of DAPK2 in NB4 APL cells lacking PU.1 partially rescues neutrophil differentiation. NB4 PU.1 knockdown cells were transduced with a control or a DAPK2-expressing lentiviral vector. Expression and knockdown efficiency were measured by Western blot (A) or qPCR (B). Cells were treated for 6 days with 1 μM ATRA. (C and D) Increased expression of the CEBPE (C) or neutrophil marker CD11b (D) in DAPK2-rescued NB4 PU.1 knockdown (KD) cells, treated with ATRA for 4 and 6 days. Analysis as in A. MWU: *P < 0.05; **P < 0.01; ***P < 0.001. (E) May-Grünwald Giemsa staining of cytospin cells was performed. A minimum of 100 cells was counted in three optical fields of at least two independent experiments.