Skip to main content
. 2014 Jan;95(1):9–18. doi: 10.1189/jlb.1112587

Figure 3. Validation of gene expression arrays demonstrates a role for Mmp28 in limiting M1 and promoting M2 phenotypes.

Figure 3.

(A) Fold increase in inflammatory gene expression for Il6, Cxcl1, and Cxcl2 in WT (black bars) and Mmp28−/− (white bars) BMDMs at 24 h post-LPS stimulation compared with untreated, M0 controls (n=3–6/genotype/condition). (B) Protein levels of secreted inflammatory mediators, TNF-α, IL-1β, CXCL2, and MCP-1 in WT and Mmp28−/− M1 cells (n=6/genotype). (C) Caspase 3/7 activity in WT and Mmp28−/− M0 and M1 cells, demonstrating increased activity in Mmp28−/− M1 cells at 48 h post-LPS (n=5–10/genotype). (D) Fold increase in gene expression of M2 markers, Fizz1, Arg1, and Il10 in WT and Mmp28−/− at 48 h post-treatment with IL-4 and IL-13 (n=6/genotype). *P < 0.05.