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. 2013 Nov 19;4:2773. doi: 10.1038/ncomms3773

Figure 4. Activated platelets induced to secrete FVIII:C.

Figure 4

To detect FVIII:C activity present in lysate of quiescent, untreated platelets (black circle; (−) agonist) and FVIII:C activity remaining in lysate after secretion of BDDFVIII from platelets treated with a mixture of physiological agonists of platelet activation: ADP, epinephrine and canine PAR1,3,4 (white circle: (+) agonist). Platelet lysates from a FVIII-deficient negative control dog show that the level of FVIII:C background activity is virtually unchanged for untreated and activated platelets. Dogs that received BDDFVIII-transduced G-PBC show an appreciable decrease in FVIII:C activity only after platelet activation; thus demonstrating that platelets isolated from experimental dogs can be induced to secrete FVIII. Each data point represents the mean value of FVIII:C activity from two independent samples each measured in duplicate with s.d. represented by black error bars. Shown is the result from one experiment performed at 2.7 (F20), 1.6 (I42) and 0.6 (M64) years after infusion of FVIII-transduced G-PBC, which is representative of the outcome of three separate experiments.