Table 2.
Modela | Vmax (RFU) | Km (μM) | C |
---|---|---|---|
green |
1.47*106 |
2.59*104 |
1 |
red |
2.02*104 |
373.38 |
1 |
blue | 1.87*104 | 317.71 | 1.93 |
Model green is estimated based on fluorescence study for SIAp3. Models red and blue are estimated based on SIAp4 data.
C, Hill coefficient; Km, aptamer concentration required to have one-half of the maximum fluorescence; RFU, relative fluorescence units; Vmax, maximum fluorescence at equilibrium.