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. 1986 Oct;83(20):7879–7882. doi: 10.1073/pnas.83.20.7879

Immune responses to H-2Kd antigen expressed by recombinant vaccinia virus.

B E Coupar, M E Andrew, D B Boyle, R V Blanden
PMCID: PMC386826  PMID: 3489941

Abstract

A recombinant vaccinia virus (VV-H2Kd-6) containing the coding sequence for the murine major histocompatibility complex class I antigen H-2Kd has been constructed and used to express H-2Kd on the surface of infected cells. Vaccinia expressed H-2Kd has been shown to generate an H-2Kd-specific primary cytotoxic T-cell response in mice infected with the recombinant virus and to stimulate an H-2Kd-specific cytotoxic T-cell response in vitro. Cells infected with the recombinant virus acted as targets for specific lysis by appropriate alloreactive cytotoxic T cells, albeit relatively inefficiently when compared with alloreactive recognition and specific lysis of H-2Kd-containing P815 cells. However, H-2Kd expressed by the recombinant virus was recognized efficiently as a restricting element in association with vaccinia virus antigens, while lysis of VV-H2Kd-6-infected L929 (H-2k) cells by CBA/H (H-2k) anti-C3H.OH (H-2KdDk) cytotoxic T cells was comparatively weak. These data suggest that there are quantitative or qualitative differences, or both, between H-2Kd expressed by vaccinia virus and cells of the H-2d haplotype. Qualitative differences have not been demonstrated but cannot be excluded.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Askonas B. A., Mullbacher A., Ashman R. B. Cytotoxic T-memory cells in virus infection and the specificity of helper T cells. Immunology. 1982 Jan;45(1):79–84. [PMC free article] [PubMed] [Google Scholar]
  2. Bennink J. R., Yewdell J. W., Smith G. L., Moller C., Moss B. Recombinant vaccinia virus primes and stimulates influenza haemagglutinin-specific cytotoxic T cells. Nature. 1984 Oct 11;311(5986):578–579. doi: 10.1038/311578a0. [DOI] [PubMed] [Google Scholar]
  3. Blanden R. V., Kees U., Dunlop M. B. In vitro primary induction of cytotoxic T cells against virus-infected syngeneic cells. J Immunol Methods. 1977;16(1):73–89. doi: 10.1016/0022-1759(77)90040-0. [DOI] [PubMed] [Google Scholar]
  4. Boyle D. B., Coupar B. E., Both G. W. Multiple-cloning-site plasmids for the rapid construction of recombinant poxviruses. Gene. 1985;35(1-2):169–177. doi: 10.1016/0378-1119(85)90169-6. [DOI] [PubMed] [Google Scholar]
  5. Hurwitz J. L., Korngold R., Doherty P. C. Specific and nonspecific T-cell recruitment in viral meningitis: possible implications for autoimmunity. Cell Immunol. 1983 Mar;76(2):397–401. doi: 10.1016/0008-8749(83)90383-0. [DOI] [PubMed] [Google Scholar]
  6. Lalanne J. L., Delarbre C., Gachelin G., Kourilsky P. A cDNA clone containing the entire coding sequence of a mouse H-2Kd histocompatibility antigen. Nucleic Acids Res. 1983 Mar 11;11(5):1567–1577. doi: 10.1093/nar/11.5.1567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MacDonald H. R., Cerottini J. C., Ryser J. E., Maryanski J. L., Taswell C., Widmer M. B., Brunner K. T. Quantitation and cloning of cytolytic T lymphocytes and their precursors. Immunol Rev. 1980;51:93–123. doi: 10.1111/j.1600-065x.1980.tb00318.x. [DOI] [PubMed] [Google Scholar]
  8. Mackett M., Smith G. L., Moss B. General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes. J Virol. 1984 Mar;49(3):857–864. doi: 10.1128/jvi.49.3.857-864.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mackett M., Smith G. L., Moss B. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415–7419. doi: 10.1073/pnas.79.23.7415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moss B. Inhibition of HeLa cell protein synthesis by the vaccinia virion. J Virol. 1968 Oct;2(10):1028–1037. doi: 10.1128/jvi.2.10.1028-1037.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Müllbacher A. Neonatal tolerance to alloantigens alters major histocompatibility complex-restricted response patterns. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7689–7691. doi: 10.1073/pnas.78.12.7689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ozato K., Mayer N. M., Sachs D. H. Monoclonal antibodies to mouse major histocompatibility complex antigens. Transplantation. 1982 Sep;34(3):113–120. doi: 10.1097/00007890-198209000-00001. [DOI] [PubMed] [Google Scholar]
  13. Panicali D., Davis S. W., Weinberg R. L., Paoletti E. Construction of live vaccines by using genetically engineered poxviruses: biological activity of recombinant vaccinia virus expressing influenza virus hemagglutinin. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5364–5368. doi: 10.1073/pnas.80.17.5364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Panicali D., Paoletti E. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4927–4931. doi: 10.1073/pnas.79.16.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Paoletti E., Lipinskas B. R., Samsonoff C., Mercer S., Panicali D. Construction of live vaccines using genetically engineered poxviruses: biological activity of vaccinia virus recombinants expressing the hepatitis B virus surface antigen and the herpes simplex virus glycoprotein D. Proc Natl Acad Sci U S A. 1984 Jan;81(1):193–197. doi: 10.1073/pnas.81.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pimsler M., Forman J. Estimates of the precursor frequency of cytotoxic T lymhocytes against antigens controlled by defined regions of the H-2 gene complex: comparison of the effect of H-2 differences due to intra-H-2 recombination vs mutation. J Immunol. 1978 Oct;121(4):1302–1305. [PubMed] [Google Scholar]
  17. Rhim J. S., Cho H. Y., Huebner R. J. Non-producer human cells induced by murine sarcoma virus. Int J Cancer. 1975 Jan 15;15(1):23–29. doi: 10.1002/ijc.2910150104. [DOI] [PubMed] [Google Scholar]
  18. Ryser J. E., MacDonald H. R. Limiting dilution analysis of alloantigen-reactive T lymphocytes. I. Comparison of precursor frequencies for proliferative and cytolytic responses. J Immunol. 1979 May;122(5):1691–1696. [PubMed] [Google Scholar]
  19. Skinner M. A., Marbrook J. An estimation of the frequency of precursor cells which generate cytotoxic lymphocytes. J Exp Med. 1976 Jun 1;143(6):1562–1567. doi: 10.1084/jem.143.6.1562. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith G. L., Godson G. N., Nussenzweig V., Nussenzweig R. S., Barnwell J., Moss B. Plasmodium knowlesi sporozoite antigen: expression by infectious recombinant vaccinia virus. Science. 1984 Apr 27;224(4647):397–399. doi: 10.1126/science.6200932. [DOI] [PubMed] [Google Scholar]
  21. Smith G. L., Mackett M., Moss B. Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen. Nature. 1983 Apr 7;302(5908):490–495. doi: 10.1038/302490a0. [DOI] [PubMed] [Google Scholar]
  22. Smith G. L., Murphy B. R., Moss B. Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters. Proc Natl Acad Sci U S A. 1983 Dec;80(23):7155–7159. doi: 10.1073/pnas.80.23.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wiktor T. J., Macfarlan R. I., Reagan K. J., Dietzschold B., Curtis P. J., Wunner W. H., Kieny M. P., Lathe R., Lecocq J. P., Mackett M. Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7194–7198. doi: 10.1073/pnas.81.22.7194. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. de la Salle H., Altenburger W., Elkaim R., Dott K., Dieterlé A., Drillien R., Cazenave J. P., Tolstoshev P., Lecocq J. P. Active gamma-carboxylated human factor IX expressed using recombinant DNA techniques. Nature. 1985 Jul 18;316(6025):268–270. doi: 10.1038/316268a0. [DOI] [PubMed] [Google Scholar]

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