Figure 3. Fit with the C4L-*O4L model including ligand unbinding and deactivation.

(a) Scheme of the C4L-*O4L model. C_x and O_x (n=0…4) denote closed and open states, respectively. *O_x denotes states of an extra pathway used only in deactivation. L is a ligand. Again, the numbers provide the values of the rate constants (s⁻¹ or s⁻¹M⁻¹) determined by the fit. Rate constants set equal in the fit are indicated by using the same colours. The errors for each rate constant are given in Supplementary Table S2. (b) The time courses of ligand binding and unbinding as well as activation and deactivation are the same as those in Fig. 2b. (c) The steady-state relationships are the same as those in Fig. 2c. Each data point is the mean of measurements obtained from 6 to 15 patches. Error bars denote s.e.m. The red curves in b and c are the result of the best global fit with the C4L-*O4L model. The reduced χ_r² was 6.0.