FIG. 6.

Hypothermia decreased the overall triggering receptor expressed on myeloid cells-2 (TREM2) expression, but increased the proportion of TREM2-positive macrophages. Representative photographs of TREM2-positive cells at days 7 (A) NT, (B) delayed normothermia, (C) eHT, 14 (D) NT, (E) delayed normothermia, and (F) eHT. (G) TREM2 expression among the NT group showed little to no staining at day 1, but markedly increased by day 3, peaking at day 7, and with a few positive cells remaining by 30 days. Hypothermia, whether early or delayed, decreased the numbers of TREM2-positive cells at day 3 and 14, but little difference was noticed at day 7 and 30. (H) TREM2 counts normalized to total IB4 counts suggest that the proportion of TREM2 microglia/macrophages is higher in the eHT group compared with the other two groups at day 7 (*p<0.01). Scale bar=40 μm. Color images available online at www.liebertpub.com/ther