FIG. 8.

Immunostaining for 60 kD heat shock protein (HSP60), the proposed ligand for TREM2 is unaffected by hypothermia. DAB staining for HSP60 revealed that ischemic insult upregulates its expression (A; sham, B; ischemic penumbra at day 7). However, neither cooling paradigm changed the numbers of HSP60 positive at any time point (C). Brains were double labeled for HSP60 (red, E, I) plus a neuron (MAP2, D) or astrocyte (GFAP, H) marker, and counterstained with DAPI (F, J). HSP60 was present in both neurons (G) and astrocytes (K). Stains from day 7 are shown. Color images available online at www.liebertpub.com/ther