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Protective efficacy after lethal challenge infection. Lung samples from individual mice in each group (n=6) were collected on day 4 postchallenge with a lethal dose of mouse-adapted A/PR/8/34 (10X LD50). Each lung sample was diluted to 1 mL with Dulbecco's modified Eagle's medium. (A) Lung virus titer; (B) Body weight changes; (C) Survival rates.
