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The ZiP-TAG™ conversion architecture. A target-specific biotinylated primer is extended with dGTP, dATP, dZTP, and dTTP to create a GAZT extension sequence (step 1). The biotinylated product is captured by a color-coded Luminex microsphere via hybridization of bead-bound CTPA probe with its complementary GAZT extension (step 2). The captured product is labeled with fluorescent streptavidine-phycoerythrin (SAPE) and analyzed by Luminex cytometer (steps 3 and 4).
