FIG. 5.
Cell cycle analysis was performed in OFs, to assess PGF2α effects, presented as scatter plots (A, C) and histograms (B, D) in the control (A, B), and 10−6 M PGF2α-treated (C, D) cells. The x-axis on the scatter plot represents cell size where the arbitrary box was drawn to gate single cells, and the y-axis represents fluorescence intensity of DNA dye (propidium iodide). The histograms show the G1, S, and G2/M (figures above=percent of cells) phases in (B) control (+DMSO) medium or (D) treated with PGF2α. The histogram reports DNA content (x-axis) and cell number (y-axis). The figure presents data from Graves' OFs and is a representative experiment of two performed (using OFs from different donors), both in duplicate.