Release of myeloid lineage cells into circulation–determination of optimal dose of rmLF. Mice were given rmLF in the following doses: 25, 100, 500, and 2,500 μg. Samples of blood were taken 24 h after administration of LF. The results are presented as the mean value of five mice per group. Statistics: Bands: control versus rmLF 25, P<0.02; control versus rmLF 100, P<0.0002; control versus rmLF 500, P<0.0002; control versus rmLF 2,500, P<0.01; neutrophils: control versus rmLF 25, NS (P=0.99); control versus rmLF 100, P<0.002; control versus rmLF 500, P<0.05; control versus rmLF 2,500, NS (P=0.9); eosinophils: control versus rmLF 25, P<0.02; control versus rmLF 100, P<0.02; control versus rmLF 500, P<0.0005; control versus rmLF 2,500, P<0.0002; lymphocytes: control versus rmLF 25, NS (P=0.24); control versus rmLF 100, P<0.0002; control versus rmLF 500, P<0.0002; control versus rmLF 2,500, P<0.0005; monocytes: control versus rmLF 25, NS (P=1.0); control versus rmLF 100, NS (P=1.0); control versus rmLF 500, NS (P=0.083); control versus rmLF 2,500, NS (P=0.083). B, bands; Ne, neutrophils; E, eosinophils; L, lymphocytes; M, monocytes.