FIG. 2.

Migration of MSCs to joints of mice with CIA. (A) CIA was induced in DBA/1 mice as described. MSCs were delivered intravenously on day 21 or 32. Mice were sacrificed 5 and 14 days after delivery of cells and organs were harvested for DNA isolation and PCR analysis. (B) PCR products after amplification of positive controls were run on agarose gel to confirm the product sizes of eGFP and GAPDH [base pair (bp)]. The detection of eGFP was used to determine the presence of DNA from MSCs isolated from eGFP transgenic mice on an FVB background. (C, D) Only trace amounts of eGFP DNA were detected in the joints of mice with no arthritis. When MSCs were delivered intravenously to symptomatic mice at day 21 or 32 they were detected predominantly in the knee 5 and 14 days later and to a lesser extent in the paw. (D) eGFP was also detected in the lung and the liver 5 and 14 days after injection. The highest levels were found in the liver of mice who had MSCs injected during the period of overt clinical disease (day 32). Results shown are mean values with two mice per treatment group. CIA, collagen induced arthritis; FVB, unbred mouse strain.