FIG. 1.

Generation of ECFCs. (A) Mononuclear cells from cord blood were transformed into spindle-like ECFCs after they were cultured for 14 days and formed colonies. (B) These ECFCs (a) uptake DiI-acLDL and (b) bind FITC-UEA-1 (c, two-color overlay). (C) ECFCs bind antibodies against the stem cell marker, CD34, and the endothelial cell markers, KDR, vWF, and VE-Cad. Nuclei were counterstained with DAPI. The image panels are representative of three separate experiments. ECFCs, endothelial colony-forming cells; Dil-acLDL, DiI-labeled acetylated low-density lipoprotein; FITC, fluorescein isothiocyanate; UEA, Ulex europeus agglutinin; KDR, kinase domain receptor; vWF, von Willebrand factor; VE-Cad, vascular endothelial cadherin; DAPI, 4’,6-diamidino-2-phenylindole. Color image is available online at www.liebertpub.com/neu