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. 2013 Aug 7;22(24):3252–3260. doi: 10.1089/scd.2013.0013

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 5. — Quantification of three germ layer marker expression by qRT-PCR in EBs treated with EC-CM, rhBMP-2, rhBMP-4, NOG, CHRDL1, or combinations. (a) CD31. (b) cTnI. (c) Nestin. (d) NF-L. (e) PDX-1. (f) Insulin1. Increases in relation to control (eg, EB control vs. EB+EC-CM) are represented by “*.” Decreases in relation to the conditioned media (eg, EB+EC-CM vs. EB+EC-CM+NOG) are represented by “**.” Decreases in relation to BMP-2 (eg, EB+BMP-2 vs. EB+BMP-2+NOG) effects are represented by “.” Decreases in relation to BMP-4 (eg, EB+BMP-4 vs. EB+BMP-4+CHRDL1) effects are represented by “.” *p≤0.05, **p≤0.05, p≤0.01, p≤0.01. NOG, noggin; CHRDL1, chordin-like-1; cTnI, cardiac troponin 1.

Inline graphic — Quantification of three germ layer marker expression by qRT-PCR in EBs treated with EC-CM, rhBMP-2, rhBMP-4, NOG, CHRDL1, or combinations. (a) CD31. (b) cTnI. (c) Nestin. (d) NF-L. (e) PDX-1. (f) Insulin1. Increases in relation to control (eg, EB control vs. EB+EC-CM) are represented by “*.” Decreases in relation to the conditioned media (eg, EB+EC-CM vs. EB+EC-CM+NOG) are represented by “**.” Decreases in relation to BMP-2 (eg, EB+BMP-2 vs. EB+BMP-2+NOG) effects are represented by “.” Decreases in relation to BMP-4 (eg, EB+BMP-4 vs. EB+BMP-4+CHRDL1) effects are represented by “.” *p≤0.05, **p≤0.05, p≤0.01, p≤0.01. NOG, noggin; CHRDL1, chordin-like-1; cTnI, cardiac troponin 1.