FIG. 1.

Porcine retinal transduction after AAV subretinal delivery. LW pigs were subretinally injected with 1×10¹⁰ GC/eye of AAV2/5 (a), 2/8 (b), or 2/9-CMV-EGFP (c). Retinal cryosections were obtained 4 weeks after injection, and EGFP was analyzed using fluorescence microscopy. (d) Fluorescence intensity in the PR layer was quantified for each group of animals; p=0.3, one-way ANOVA. Scale bar=25 μm. AAV, adeno-associated virus; ANOVA, analysis of variance; AU, arbitrary units provided by the LAS AF lite software (see Materials and Methods); CMV, cytomegalovirus; EGFP, enhanced green fluorescent protein; LW, Large White; n, number of eyes; ONL, outer nuclear layer; PR, photoreceptor; RPE, retinal pigment epithelium. Color images available online at www.liebertpub.com/hum