FIG. 2.

AAV-mediated cone transduction in pig retina. LW pigs were subretinally injected with AAV2/5, 2/8, and 2/9-CMV-EGFP (1×10¹⁰ GC/eye of each vector). (a) Retinal cryosections were obtained 4 weeks after injection, and anti-LUMIf-hCAR immunostaining (red) was performed to label cones; EGFP+ cones are indicated by white arrows. (b) Higher magnification of the transduced cones. The confocal microscopy images are representative of single plans of Z-stack taken at 63×magnification. (c) Quantification of cone transduction efficiency of AAV2/5, 2/8, and 2/9-CMV-EGFP. The histograms represent the percentage of EGFP+/CAR+ cells counted on a total of 150–200 CAR+ cells. p=0.63, one-way ANOVA. CAR, cone arrestin; DAPI, 4′,6′-diamidino-2-phenylindole; INL, inner nuclear layer.