FIG. 3.

AAV2/8-mediated retinal transduction in pig retina. LW pigs were subretinally injected with AAV2/8-CMV-EGFP (a), AAV2/8-CMV-EGFP-miR204T (b), and AAV2/8-GRK1-EGFP (c, 1×10¹⁰ GC/eye of each vector). Retinal cryosections were obtained 4 weeks after injection, and EGFP was analyzed using fluorescence microscopy. The exposure time is the same for the eyes injected with AAV2/8-CMV-EGFP and AAV2/8-CMV-EGFP-miR204T, while for AAV2/8-GRK1-EGFP the representative image was taken at an exposure time that was double the other vectors. (d) Fluorescence intensity in the PR layer was quantified for each group of animals at the same exposure time; p=0.13, one-way ANOVA. Scale bar=25 μm. Color images available online at www.liebertpub.com/hum