FIG. 6.

Retinal function assessment in Gucy2e^−/− mice after AAV2/8-mediated gene transfer. Gucy2e^−/− mice were treated at P10 with 1.5×10⁹ GC/eye of either AAV2/8-GUCY2D in one eye or AAV2/8-EGFP (or untreated) in the contralateral eye. Six-hertz flicker results of Gucy2e^−/− animals treated with either AAV2/8-CMV-GUCY2D (a) or AAV2/8-GRK1-GUCY2D (b) were analyzed 1 month postinjection. (c) Retinal function was also assessed by paired flash ERG. The histograms represent the average amplitudes of the responses to either the first (black bar) or the second (white bar) flash. n is the ratio between the number of represented eyes and the total number of injected eyes. The numbers of represented eyes in the CMV and GRK1 groups are those in which improvement of cone activity was evident, which is additionally indicated as % of total in parentheses. *p≤0.05; **p≤0.01; ***p≤0.001, one-way ANOVA.