Table 3.
Testing the gene duplication hypothesis: molecular phenotype frequencies for the AaNav 1011 site in F1 offspring from crossings between Ae. aegypti Ile/Ile X Ile/Met
| Crossings | F1 observed (n) |
Hypothesesa |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Without duplication |
With duplication |
||||||||||
| Hypothesis 1 |
Hypothesis 2a |
Hypothesis 2b |
|||||||||
| Ile/Ile | Ile/Met | Ile/Ile | Ile/Met | P | Ile/Ile | Ile/Met | P | Ile/Ile | Ile/Met | P | |
| #1 (♀ Ile/Met x ♂ Ile/Ile) | 0 | 20 | 10 | 10 | *** | 0 | 20 | NS | 10 | 10 | *** |
| #2 (♀ Ile/Met x ♂ Ile/Ile) | 0 | 20 | 10 | 10 | *** | 0 | 20 | NS | 10 | 10 | *** |
| #3 (♀ Ile/Met x ♂ Ile/Ile) | 8 | 12 | 10 | 10 | NS | 0 | 20 | ** | 10 | 10 | NS |
| #4 (♀Ile/Ile x ♂ Ile/Met) | 9 | 9 | 9 | 9 | NS | 0 | 18 | *** | 9 | 9 | NS |
| #5 (♀ Ile/Ile x ♂ Ile/Met) | 0 | 30 | 15 | 15 | *** | 0 | 30 | NS | 15 | 15 | *** |
| #6 (♀ Ile/Met x ♂ Ile/Ile) | 0 | 30 | 15 | 15 | *** | 0 | 30 | NS | 15 | 15 | NS |
| #7 (♀ Ile/Met x ♂ Ile/Ile) | 0 | 22 | 11 | 11 | *** | 0 | 22 | *** | 11 | 11 | NS |
Molecular phenotype frequencies were determined by AS-PCR for the AaNaV 1011 site (see ‘Materials and Methods’ section). aExpected numbers of F1 individuals of each molecular phenotype based on the three hypotheses of parental haplotype constitution (Fig. 3). Significance of the deviations of the tested hypotheses obtained through Fisher’s exact test: NS = non-significant, **P < 0.01, ***P < 0.001.