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. Author manuscript; available in PMC: 2013 Dec 19.
Published in final edited form as: Dev Biol. 2010 Dec 23;351(1):10.1016/j.ydbio.2010.12.025. doi: 10.1016/j.ydbio.2010.12.025

Fig 2. Loss ofE2f1-3 causes transcriptional deregulation of a subset of lens-specific genes.

Fig 2

(A) Immunostaining of α-, β-, and γ-crystallin in control and cry-cre lenses. The distribution of positive staining ranges from the anterior face of the lens to the transition zone. β- and γ-crystallins are only detectable in the fiber cell compartment. Low magnification images of immunostaining with α-, β-, and γ-crystallin antibodies shows specificity for lens. Surrounding retina provides reference for background levels of fluorescence. (B) Lens differentiation markers were analyzed by hierarchical clustering of gene expression and visualized by heatmap. Statistically significant changes (p < 0.001) were determined by class comparison analysis and are indicated by red boxes (upregulated) and green boxes (downregulated).