Table 3. Effects of p.D1816VfsX7 mutation on time-, and voltage-dependent Nav1.5 channel properties.
| Nav1.5 construct |
Activation
|
Inactivation
|
Reactivation
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Vh (mV) | k (mV) | Time to peak (ms) | Vh (mV) | k (mV) | τf (ms) | τs (ms) | τf (ms) | τs (ms) | |
| WT | -45.2±1.4 | 5.6±0.4 | 0.53±0.03 | -82.1±1.9 | 6.5±0.3 | 0.9±0.1 | 5.7±0.3 | 3.5±0.6 | 18.8±2.4 |
| p.D1816VfsX7 | -27.3±2.1* | 5.4±0.4 | 0.28±0.02* | -61.5±2.5* | 6.0±1.0 | 0.8±0.1 | 4.7±0.6 | 1.5±0.3* | 24.5±3.4 |
| p.D1816VfsX7+WT | -38.5±6.6* # | 5.5±0.5 | 0.54±0.06 | -69.6±6.5* # | 6.1±0.4 | 1.1±0.1 | 5.8±1.0 | 2.2±0.4* # | 17.9±4.0 |
Vh and k: midpoint and slope values of the activation and inactivation curves. τf and τs: fast and slow time constants of inactivation or reactivation
Values are mean±SEM of >10 experiments. * P<0.01 vs WT. # P<0.01 vs p.D1816VfsX7