Figure 2.

Effects of si-SCN2A vector transfection on Cu-Zn superoxide dismutase (SOD) expression and resting membrane electric potential. (A and B) Downregulated transcript and protein expression levels of of SCN2A following transfection with the si-SCN2A vector, respectively. (C) No significant signals were observed in the si-SCN2A vector groups by immunofluorescence. (D) Cu-Zn SOD concentrations among the control, empty vector and si-SCN2A vector groups. Different letters (a or b) show significant differences among the groups (P<0.05). (E) Resting membrane electric potential detected by patch clamp showed a repeated discharge caused by si-SCN2A.