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LGR5 knockdown suppresses plate colony formation. Equal numbers of parental U87, U87-NC and U87-KD cells were seeded onto 6-well plates. (A) The cells were fixed and stained with Giemsa after 14 days. (B) The number of colonies in U87-KD cells was significantly less than that in the U87-NC and non-transfected U87 cells (*P<0.05). CON, parental U87 cell; NC, negative control (shRNA-Ctr/U87); KD, LGR5 knockdown cells (shRNA-LGR5/U87).
