Transgenic expression of Bcl-3 in B cells reduces MZ and increases FO B cells. (A, B) Representative flow cytometric analyses of splenocytes from 6–8-week-old WT, KO and Bcl-3transgenic (Tg) mice stained for expression of surface markers with gates across top of panels as indicated. Percentages of various subpopulations are shown; fractions (FR) I, II and III in (B) refer to subpopulations shown in first row of panels. (FO I, II = follicular 1, 2; other abbreviations as in Fig.1) Data for (A, B) representative of 3 separate experiments with n=7–9 independently analyzed mice per group. (C) Summary of data as in (B) shown as mean ±SEM (*= p<0.05). Left panel, percentages of B-cell subpopulations; center panel, total number of splenic B cells for each genotype; right panel, total numbers of B-cell subpopulations.