Table VI.

Validation of miRNA expression profiles by qRT-PCR.

miRNA	Group	CtU6	CtmiRNA	ΔCt = CtmiRNA − CtU6	Ct(MN − CG) = ΔCtMN − ΔCtNC	2−ΔΔC a	MN/NC ratiob	log2 (MN/NC ratio)c
hsa-miR-7-5p	NC	10.88	21.61	10.73	0	1	0.03	−5.06
	MN	11.37	27.16	15.79	5.06	0.03
hsa-miR-615-3p	NC	10.88	17.26	6.38	0	1	0.19	−2.40
	MN	11.37	20.15	8.78	2.40	0.19
hsa-miR-577	NC	10.88	35.40	24.52	0	1	0.46	−1.12
	MM	11.37	37.01	25.64	1.12	0.46
hsa-miR-98	NC	10.88	28.04	17.16	0	1	19.43	4.28
	MN	11.37	24.25	12.88	−4.28	19.43
hsa-miR-375	NC	10.88	19.51	8.63	0	1	6.19	2.63
	MN	11.37	17.37	6.00	−2.63	6.19

^a2^−ΔΔCt value, used to determine changes in expression levels in MN samples relative to NC samples. 2^−ΔΔCt = 2^{−Ct(MN − NC)}.

^b2^−ΔΔCt(MN)/2^−ΔΔCt(NC).

^cEquivalent to the fold change (log₂) value provided in the microarray analysis of miRNA expression in the MN and NC groups.

If c <0, miRNA expression was downregulated; if c >0, miRNA expression was upregulated. miRNA, microRNA; MN, membranous nephropathy; NC, normal controls.