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Western blot analysis for p15 protein in HCT116 cells. (A) HCT116 cells were treated with 1 mg/ml or 2 mg/ml of antineoplaston AS2-1 for up to 48 h. (B) HCT116 cells were treated with 0.2, 0.5, 1 and 2 mg/ml of AS2-1 for 12 or 24 h. AS2-1 upregulated the expression of p15 protein in a time- and dose-dependent manner in HCT116 cells.
