Table 1. Primers used for microsatellite genotyping, and genomic localization.

Primers	Sequence	Genome Localization	Gene	Reference
SmBr5	For: GAATTACTGTCCCTTTATCTC	Smp_scaff000422	Smp-171310 gene
	Rev: AAACTATTCATTACTGTCGGG	Location: 224437–224454	Hypothetical protein	42
SmBr6	For: CTTAACAGACATACACGC	Smp_scaff000003	Intergenic region	42
	Rev: GAATACAGGCTATAATCTACA	Location: 1550379–1550637
13TAGA	For: GTACATTTTATGTCAGTTAGCC	Smp_scaff000419	Smp_089460 gene	43
	Rev: CATGATCTTAGCTCAGAGAGC	Location: 158262–158282	Calpain B, putative
SmBr9	For: ATTCACCCATTGTCTTAAAACC	Smp_scaff000316	Intergenic region	43
	Rev: ATTGGCGTCAGTAGAAGAGATT	Location: 14607–14767
SmBr13	For: GTCACAGATACCTGACGAGCTG	Smp_scaff000328	Intergenic region	43
	Rev: ACTCCCCAGCAATTTGTCC	Location: 185390–185611

The genomic localization of microsatellites was identified using Blast Search in SchistoDB [54].